Expression, Purification, and Characterization of a Recombinant Neutral Ceramidase fromMycobacterium tuberculosis
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چکیده
منابع مشابه
Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis.
Ceramidase (CDase) catalyzes the hydrolysis of ceramide (Cer) to sphingosine (Sph) and fatty acid. We have reported the molecular cloning and preliminary characterization of the Mycobacterium CDase (MtCDase) (J. Biol. Chem., 274, 36616-36622 (1999)). To determine its function further, MtCDase was expressed in Escherichia coli and purified by Ni-Sepharose and gelfiltration. The purified recombin...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2010
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.90645